Going beyond the single nano region and into the sub-nano region, the IG-1000 uses the induced grating (IG) method, which is based on a new principle for measuring the size of nanoparticles using the phenomenon of dielectrophoresis and diffracted light, to provide excellent reproducibility and the acquisition of stable data, particularly for sub-10 nm particles.
The IG method does not use scattered light, is free from physical restrictions and does not require the input of the refractive index as a measurement condition. As a result, the IG-1000 can measure the size of nanoparticles simply and with high sensitivity, and is particularly effective for the analysis of single nanoparticles.
High-Sensitivity Analysis of Single Nanoparticles
Stable measurement with good reproducibility is possible because
the IG-1000 utilizes optical signals emitted by the diffraction
grating formed by the particles, and not scattered light emitted by
the particles. Even in the single nano region, a good S/N ratio can
be obtained.
Resistance to Contamination
This new measurement principle is resistant to contamination and,
even if the sample is mixed with small amounts of foreign
particles, information about the particles to be analyzed is
captured reliably. The filtering of samples in order to remove
coarse particles is not required.
High Reproducibility
The IG method ensures high reproducibility and the acquisition of
stable data. In particular, high reproducibility for particle sizes
of less than 10 nm removes the uncertainty of particle analysis in
the single nano region. The viewing of the raw data of diffracted
light is also possible, enabling a simple approximate validation of
measurement results.
Simple, Three-step Workflow
With the IG-1000, analysis is easy: inject the sample, insert the
electrode and begin analysis. Using the IG method, it can measure
particles in the 0.5 to 200 nm range in about 30 seconds, from
measurement start to displayed results.
Particle size is measured using the diffusion rate of a grating which is composed of particles in the liquid. The diffusion rate of large particles is slow and that of small particles, especially nanometers particles, is fast. The diffusion behavior of particles can be monitored by detecting the change of primary diffracted light, and the concentration of nanoparticles expresses itself as a change in the refractive index.
In the IG-1000 a grating electrode concentrates the particles in a grid, and when the electrodes are turned off the diffraction grating disappears. The decay process of this particle density diffraction grating is measured via the change in intensity of the diffracted light, and a diffusion coefficient is obtained.
Diffraction Grating Consisting of Microscopic Particles Formed by
Dielectrophoresis
An alternating voltage is applied to cyclically arranged
electrodes, and a cyclic concentration distribution of microscopic
particles is formed in the liquid by dielectrophoresis. Although
the cyclic concentration distribution of microscopic particles acts
as a diffraction grating (a particle concentration diffraction
grating), if the alternating voltage is stopped, the grating
diffuses and disappears (patent pending).
Key Point of IG Method
Precision Measurement Achieved with Modification of Electrode
Configuration
The cyclically arranged electrodes also function as a diffraction
grating, although the light created is weaker than the diffracted
light created by the particle concentration diffraction grating.
The electrode configuration has been modified as shown in the
figure so that the pitch of the electrode diffraction grating is
half that of the particle concentration diffraction grating (patent
pending). In this way there is a more precise measurement.
| IG-1000 | |||
|---|---|---|---|
| Measurement Principle | Induced grating (IG) method | ||
| Measurement Range | 0.5 to 200 nm | ||
| Measurement Time | 30 sec (from the start of measurement to the display of results) | ||
| Sample Liquid V |
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